Preparation of 14C-labeled Tuberculin Purified Protein Derivative
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چکیده
منابع مشابه
Preparation of 14C-labeled tuberculin purified protein derivative.
(14)C-labeled tuberculin purified protein derivative ((14)C-tuberculin PPD) has been prepared from culture filtrates of Mycobacterium tuberculosis var. hominis grown in a culture medium containing uniformly labeled (14)C-amino acids. With a mixture of (14)C-amino acids (an acid hydrolysate of (14)C-Chlorella protein) in the medium, the recoveries of (14)C in the final product were higher than w...
متن کاملComparative study of 14C-labeled purified protein derivative from various mycobacteria. I. Preparation of 14C-labeled purified protein derivative antigens and their adsorption to glass.
Biologically active (14)C-labeled purified protein derivative ((14)C-PPD) has been prepared from the culture filtrates of seven species of mycobacteria, namely Mycobacterium tuberculosis Johnston strain (PPD), M. bovis BCG (PPD-BCG), M. avium (PPD-A), M. kansasii (PPD-Y), M. intracellulare (PPD-B), M. scrofulaceum (PPD-G), and M. fortuitum (PPD-F). These mycobacteria were grown in a culture med...
متن کاملPreparations of Tuberculin Purified Protein Derivative
LANDI, S. (University of Toronto, Toronto, Ontario, Canada), AND H. R. HELD. Physicochemical and biological studies on various preparations of tuberculin purified protein derivative. Appl. Microbiol. 13:132-139. 1965.-Tuberculin purified protein derivative (PPD) has been prepared by seven different precipitation methods from culture filtrate of Mycobacterium tuberculosis var. hominis. It was fo...
متن کاملComparative Study of 14C-Labeled Purified Protein Derivative from Various Mycobacteria
A study on skin cross-reactivity between stabilized "4C-labeled mycobacterial antigens, namely tuberculin purified protein derivative (PPD; from Mycobacterium tuberculosis), PPD-A (M. avium), PPD-Y (M. kansasii), PPD-G (M. scrofulaceum), PPD-B (M. intracellulare), and PPD-F (M. fortuitun), has been carried out in groups of guinea pigs sensitized with one of the following heat-killed mycobacteri...
متن کاملEvaluation of Immunological Parameters in Purified Protein Derivative Positive Tuberculin Workers
Objective(s): According to the occupationally risk of infection in staff workers who have direct contact with mycobacterium species, we investigated their immunological parameters and compared with healthy purified protein derivative (PPD) negative volunteers. Materials and Methods : We investigated 20 PPD positive volunteers working at Tuberculin Unit of Razi Vaccine and Serum Research Ins...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1967
ISSN: 0003-6919
DOI: 10.1128/aem.15.4.839-843.1967